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Metabolomics - Acyl Carnitine

Identification_Information:
Citation:
Citation_Information:
Originator:The Pennsylvania State University
Publication_Date:2013
Title:
Metabolomics - Acyl Carnitine
Online_Linkage: http://metabolomics.psu.edu/
Online_Linkage: http://www.datacommons.psu.edu/
Description:
Abstract: 
Metabolomics - Acyl Carnitine
Supplemental_Information: 
Fungal strains: F52 of Metarhizium bunneum, the fungal isolate used in the commercial product Met52® and Beauveria bassiana GHA from Botanigard®. 
Insect tissues: brains and mandibular muscles of Camponotus pennsylvanicus collected in Central Pennsylvania.
Growth media: Schneider's media (Gibco) supplemented with 10% filter sterilized Fetal Bovine Serum (PAA Laboratories Inc.) 100 µg/mL kanamycin (Invitrogen), 100 units/mL penicillin and 100 µg streptomycin/mL (Invitrogen). PBS (Gibco) supplemented with 100 µg/mL kanamycin (Invitrogen), 100 units/mL penicillin and 100 µg streptomycin/mL (Invitrogen).
Incubation conditions: 3 days at 28C in 1 ml media in a 24 wells plate with 12 µm pore size cell culture insert (Millipore). 3 insect tissues per well, 3 mm x 3 mm square fungal mycelium as a starting point.
Metabolomics on media to measure secreted metabolites: 100 µl of the medium was mixed with 1 volume of acetonitrile for protein precipitation prior to LC-MS/MS analysis. Samples were randomized and run using the HPLC-QTOFMS (Shimadzu Prominence UFLC XR and AB Sciex 5600 quadrupole time-of-flight mass spectrometry) platform. Samples (5ul) were separated on a C18 Column (100 x 2.1 mm 1.7 um, Waters Acquity BEH) using a gradient elution program with aqueous acetonitrile (3-90%) at a flow rate of 250 ul/min.  Positive ion electrospray ionization mass spectra were acquired over the mass range 50-1250 Da in IDA (Information Dependent Acquisition) mode with one 100 ms survey scan and up to twenty 100 ms MS/MS product ion scans per duty cycle. 
Data extraction and alignment: MarkerView (AB Sciex) software Retention times between 0.00 and 16.00 min.; Subtraction Offset of 10 scans; Subtraction Mult. Factor of 1.3; Noise Treshold of 50; Min. Spectral Peak Width of 15 ppm; Min. RT Peak Width of 3 scans; Retention Time Tolerance of 0.10 min.; Mass Tolerance of 10.0 ppm; 3 required samples; Maximum of 100000 peaks. 
Data analysis: Analyses were done looking at the monoisotopic ions with an m/z between 100 and 800 and a retention time between 1 and 16 minutes.

Samples, every sample type contains 3 biological triplicates:

Sample no.	medium	antibiotics	Fungus	Ant tissue	Extracted
1	Schneider's FBS	 	 	 	supernatant
2	Schneider's FBS	Pen Strep + kanamycin 	Beauveria	 	supernatant
3	Schneider's FBS	Pen Strep + kanamycin 	Metarhizium	 	supernatant
4	Schneider's FBS	Pen Strep + kanamycin 	 	brain	supernatant
5	Schneider's FBS	Pen Strep + kanamycin 	 	muscle	supernatant
6	Schneider's FBS	Pen Strep + kanamycin 	Beauveria	brain	supernatant
7	Schneider's FBS	Pen Strep + kanamycin 	Beauveria	muscle	supernatant
8	Schneider's FBS	Pen Strep + kanamycin 	Metarhizium	brain	supernatant
9	Schneider's FBS	Pen Strep + kanamycin 	Metarhizium	muscle	supernatant
10	Schneider's FBS	Pen Strep + kanamycin 	 	 	supernatant
11	PBS	 	 	 	supernatant
12	PBS	Pen Strep + kanamycin 	 	 	supernatant
13	PBS	Pen Strep + kanamycin 	Beauveria	 	supernatant
14	PBS	Pen Strep + kanamycin 	Metarhizium	 	supernatant
15	PBS	Pen Strep + kanamycin 	 	brain	supernatant
16	PBS	Pen Strep + kanamycin 	 	muscle	supernatant
17	PBS	Pen Strep + kanamycin 	Beauveria	brain	supernatant
18	PBS	Pen Strep + kanamycin 	Beauveria	muscle	supernatant
19	PBS	Pen Strep + kanamycin 	Metarhizium	brain	supernatant
20	PBS	Pen Strep + kanamycin 	Metarhizium	muscle	supernatant
Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date:2013
Currentness_Reference: 
publication date
Keywords:
Theme:
Theme_Keyword_Thesaurus:ISO 19115 Topic Categories
Theme_Keyword:Camponotus pennsylvanicus
Theme_Keyword:biota
Access_Constraints:None.
Use_Constraints: 
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The University makes no warranty or representation, either express or implied, with respect to the FILES or accompanying documentation, including its quality, performance, merchantability, or fitness for a particular purpose. The FILES and documentation are provided "as is" and the USER assumes the entire risk as to its quality and performance.
The University will not be liable for any direct, indirect, special, incidental, or consequential damages arising out of the use or inability to use the FILES or any accompanying documentation.
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Point_of_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization:Penn State
Contact_Person:Andrew D. Patterson, PhD
Contact_Position:Assistant Professor of Molecular Toxicology
Contact_Address:
Address_Type:mailing address
Address: 
Center for Molecular Toxicology & Carcinogenesis
Address: 
Department of Veterinary & Biomedical Sciences
Address: 
322 Life Sciences Bldg
City:University Park
State_or_Province:Pennsylvania
Postal_Code:16802
Country:USA
Native_Data_Set_Environment:
Microsoft Windows 2000 Version 5.0 (Build 2195) Service Pack 4; ESRI ArcCatalog 8.3.0.800
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Distributor:
Contact_Information:
Contact_Person_Primary:
Contact_Organization:Penn State Data Commons
Contact_Address:
Address_Type:mailing and physical address
Address: 
115 Land and Water Building
City:University Park
State_or_Province:Pennsylvania
Postal_Code:16802
Country:United States
Contact_Voice_Telephone:(814) 865 - 8792
Contact_Electronic_Mail_Address:datacommons@psu.edu
Distribution_Liability: 
The USER shall indemnify, save harmless, and, if requested, defend those parties involved with the development and distribution of this data, their officers, agents, and employees from and against any suits, claims, or actions for injury, death, or property damage arising out of the use of or any defect in the FILES or any accompanying documentation. Those parties involved with the development and distribution excluded any and all implied warranties, including warranties or merchantability and fitness for a particular purpose and makes no warranty or representation, either express or implied, with respect to the FILES or accompanying documentation, including its quality, performance, merchantability, or fitness for a particular purpose. The FILES and documentation are provided "as is" and the USER assumes the entire risk as to its quality and performance. Those parties involved with the development and distribution of this data will not be liable for any direct, indirect, special, incidental, or consequential damages arising out of the use or inability to use the FILES or any accompanying documentation.
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Metadata_Reference_Information:
Metadata_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization:Penn State Data Commons
Contact_Position:Metadata Coordinator
Contact_Address:
Address_Type:mailing address
Address: 
115 Land and Water Building
City:University Park
State_or_Province:Pennsylvania
Postal_Code:16802
Country:United States
Contact_Voice_Telephone:814-865-8792
Contact_Electronic_Mail_Address:datacommons@psu.edu
Metadata_Standard_Name:FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version:FGDC-STD-001-1998
Metadata_Time_Convention:local time
Metadata_Extensions:
Online_Linkage: http://www.esri.com/metadata/esriprof80.html
Profile_Name:ESRI Metadata Profile
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