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Innate Immune Genes Associated with Newcastle Disease Virus Load in Chick Embryos from Inbred and Outbred Lines
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Citation:
Citation_Information:
Originator:The Pennsylvania State University
Publication_Date:2018
Title:
Innate Immune Genes Associated with Newcastle Disease Virus Load in Chick Embryos from Inbred and Outbred Lines
Online_Linkage: http://www.datacommons.psu.edu/
Description:
Abstract: 
Newcastle disease virus (NDV) causes substantial economic losses to smallholder farmers in low- to middle-income countries with high levels of morbidity and mortality in poultry flocks.  Previous investigations have suggested differing levels of susceptibility to NDV between specific inbred lines and amongst breeds of chickens, but the mechanisms contributing to this remain poorly understood.  Studies have shown that some of these the differences in levels of susceptibility may be accounted for by variation in the innate immune response amongst various breeds of poultry to NDV infection.  Recent studies, in inbred Fayoumi and Leghorn lines, uncovered conserved, breed-dependent, and subline-dependent responses. To better understand the role of innate immune genes in engendering a protective immune response, we assessed the transcriptional responses to NDV of three highly outbred Tanzanian local chicken ecotypes, the Kuchi, the Morogoro Medium, and the Ching’wekwe. Hierarchical clustering and principal coordinate analysis of the gene expression profiles of 21-day old chick embryos infected with NDV clustered in an ecotype-dependent manner that was consistent with the relative viral loads for each of the three ecotypes. The Kuchi and Morogoro Medium exhibit significantly higher viral loads than the Ching’wekwe.  The results show that the outbred ecotypes with increased levels of expression of CCL4, NOS2, and SOCS1 also had higher viral loads. The higher expression of SOCS1 is inconsistent with the expression in inbred lines. These differences may uncover new mechanisms or pathways in these populations that may have otherwise been overlooked when examining the response in highly inbred lines. Taken together, the results of our investigations provide insights on the specific conserved and differentially expressed innate immune-related genes involved the response of highly outbred chicken lines to NDV, and suggest that several of the specific innate immunity related genes identified in the current investigation may serve as markers for the selection of chickens with reduced susceptibility to NDV.
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Calendar_Date:2018
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Theme_Keyword_Thesaurus:ISO 19115 Topic Categories
Theme_Keyword:biota
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Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person:Vivek Kapur
Contact_Organization:Penn State
Contact_Position:Professor of Microbiology and Infectious Diseases
Contact_Address:
Address_Type:mailing address
Address: 
205 Wartik Laboratory
City:University Park
State_or_Province:Pennsylvania
Postal_Code:16802
Country:USA
Contact_Voice_Telephone:814-865-9788
Contact_Electronic_Mail_Address:vkapur@psu.edu
Contact Instructions: 
Megan A. Schilling123, Sahar Memari2, Isabella Cattadori24, Robab Katani24, Amandus P. Muhairwa5, Joram J. Buza3, Vivek Kapur1234*

1 Animal Science Department, Pennsylvania State University, University Park, PA 

2 Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA

3 School of Life Sciences and Bioengineering, The Nelson Mandela African Institution of Science and Technology, Arusha, TZ

4 Applied Biological and Biosecurity Research Laboratory, Pennsylvania State University, University Park, PA

5 Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, Morogoro, TZ
Native_Data_Set_Environment:
Microsoft Windows 2000 Version 5.0 (Build 2195) Service Pack 4; ESRI ArcCatalog 8.3.0.800
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Contact_Organization:Penn State Data Commons
Contact_Address:
Address_Type:mailing and physical address
Address: 
115 Land and Water Building
City:University Park
State_or_Province:Pennsylvania
Postal_Code:16802
Country:United States
Contact_Voice_Telephone:(814) 865 - 8792
Contact_Electronic_Mail_Address:datacommons@psu.edu
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The USER shall indemnify, save harmless, and, if requested, defend those parties involved with the development and distribution of this data, their officers, agents, and employees from and against any suits, claims, or actions for injury, death, or property damage arising out of the use of or any defect in the FILES or any accompanying documentation. Those parties involved with the development and distribution excluded any and all implied warranties, including warranties or merchantability and fitness for a particular purpose and makes no warranty or representation, either express or implied, with respect to the FILES or accompanying documentation, including its quality, performance, merchantability, or fitness for a particular purpose. The FILES and documentation are provided "as is" and the USER assumes the entire risk as to its quality and performance. Those parties involved with the development and distribution of this data will not be liable for any direct, indirect, special, incidental, or consequential damages arising out of the use or inability to use the FILES or any accompanying documentation.
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Metadata_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization:Penn State Data Commons
Contact_Position:Metadata Coordinator
Contact_Address:
Address_Type:mailing address
Address: 
115 Land and Water Building
City:University Park
State_or_Province:Pennsylvania
Postal_Code:16802
Country:United States
Contact_Voice_Telephone:814-865-8792
Contact_Electronic_Mail_Address:datacommons@psu.edu
Metadata_Standard_Name:FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version:FGDC-STD-001-1998
Metadata_Time_Convention:local time
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