Method Development Report
| MS1 Resolution | 1.0 |
|---|
| MS2 Resolution | 0.75 |
|---|
| Calibration Method | C:\MassLynx\IntelliStart\Results\Quantitative\Calibration_20120326_1.cal |
|---|
| Tune Method | C:\MassLynx\Xevo TQ-S - Acquity UPLC Installation Project.pro\ACQUDB\SYSTEM2.ipr |
|---|
| Cone Voltage Range | 2 - 100 |
|---|
| Collision Energy Range | 2 - 80 |
|---|
| Lowest Fragment Mass | 40.00 |
|---|
| Excluded Losses | 18.00, 44.00 |
|---|
Date: Generated on Thu 19 Apr 2012 at 16:11
Results
IntelliStart generated the following experiments:
| MRM Experiment |
C:\MassLynx\Xevo TQ-S - Acquity UPLC Installation Project.pro\ACQUDB\AICAR_neg.exp |
IntelliStart found the following compounds:
| Compound |
Formula/Mass |
|
Parent m/z |
Cone Voltage |
Daughters |
Collision Energy |
Ion Mode |
| AICAR |
338 |
|
|
|
|
|
|
Compound
Transition 1: ES-, m/z 337.03 -> 78.94
Cone Voltage Optimization (m/z 337.03)
 |
|
Optimized MS Spectrum (at Cone Voltage 44V)
 |
|
Collision Energy Optimization (m/z 337.03 -> 78.94)
 |
|
Optimized Daughter Spectrum (at Collision Energy 30eV)
 |
|
Transition 2: ES-, m/z 337.03 -> 96.90
Cone Voltage Optimization (m/z 337.03)
 |
|
Optimized MS Spectrum (at Cone Voltage 44V)
 |
|
Collision Energy Optimization (m/z 337.03 -> 96.90)
 |
|
Optimized Daughter Spectrum (at Collision Energy 30eV)
 |
|