Method Development Report
| MS1 Resolution | 1.0 |
|---|
| MS2 Resolution | 0.75 |
|---|
| Calibration Method | C:\MassLynx\IntelliStart\Results\Quantitative\Calibration_20120326_1.cal |
|---|
| Tune Method | C:\MassLynx\Xevo TQ-S - Acquity UPLC Installation Project.pro\ACQUDB\SYSTEM2.ipr |
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| Cone Voltage Range | 2 - 100 |
|---|
| Collision Energy Range | 2 - 80 |
|---|
| Lowest Fragment Mass | 40.00 |
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| Excluded Losses | 18.00, 44.00 |
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Date: Generated on Fri 11 May 2012 at 14:39
Results
IntelliStart generated the following experiments:
| MRM Experiment |
C:\MassLynx\Xevo TQ-S - Acquity UPLC Installation Project.pro\ACQUDB\Isocitrate_pos.exp |
IntelliStart found the following compounds:
| Compound |
Formula/Mass |
|
Parent m/z |
Cone Voltage |
Daughters |
Collision Energy |
Ion Mode |
| Isocitrate |
192 |
No transitions found. |
Compound
No parent found. Potential causes can include:
(1) Inconsistent ion beam.
(2) Poor ion intensity.
Ensure concentration of analyte is sufficient and the ion beam is stable.
Following chromatogram shows acquired data for the requested parent mass:
Cone Voltage Optimization (m/z 193.22)
 |
|
Optimized MS Spectrum (at Cone Voltage 46V)
 |
|
Potential issues include:
(1) No parent mass or poor intensity observed.
(2) Interference on parent.