Method Development Report
| MS1 Resolution | 0.75 |
|---|
| MS2 Resolution | 0.75 |
|---|
| Calibration Method | C:\MassLynx\IntelliStart\Results\Quantitative\Calibration_20120912.cal |
|---|
| Tune Method | C:\MassLynx\Xevo TQ-S - Acquity UPLC Installation Project.pro\ACQUDB\SYSTEM2.ipr |
|---|
| Cone Voltage Range | 10 - 100 |
|---|
| Collision Energy Range | 2 - 80 |
|---|
| Lowest Fragment Mass | 40.00 |
|---|
| Excluded Losses | 18.00 |
|---|
Date: Generated on Thu 06 Dec 2012 at 16:27
Results
IntelliStart generated the following experiments:
| MRM Experiment |
C:\MassLynx\Xevo TQ-S - Acquity UPLC Installation Project.pro\ACQUDB\Tetrodotoxin_pos.exp |
IntelliStart found the following compounds:
| Compound |
Formula/Mass |
|
Parent m/z |
Cone Voltage |
Daughters |
Collision Energy |
Ion Mode |
| Tetrodotoxin |
319 |
|
| 319.90 | | 319.90 | | 319.90 | | 319.90 | | 319.90 |
|
|
| 161.96 | | 284.05 | | 134.18 | | 178.16 | | 256.02 |
|
|
|
Compound
Transition 1: ES+, m/z 319.90 -> 161.96
Cone Voltage Optimization (m/z 319.90)
 |
|
Optimized MS Spectrum (at Cone Voltage 16V)
 |
|
Collision Energy Optimization (m/z 319.90 -> 161.96)
 |
|
Optimized Daughter Spectrum (at Collision Energy 38eV)
 |
|
Transition 2: ES+, m/z 319.90 -> 284.05
Cone Voltage Optimization (m/z 319.90)
 |
|
Optimized MS Spectrum (at Cone Voltage 16V)
 |
|
Collision Energy Optimization (m/z 319.90 -> 284.05)
 |
|
Optimized Daughter Spectrum (at Collision Energy 24eV)
 |
|
Transition 3: ES+, m/z 319.90 -> 134.18
Cone Voltage Optimization (m/z 319.90)
 |
|
Optimized MS Spectrum (at Cone Voltage 16V)
 |
|
Collision Energy Optimization (m/z 319.90 -> 134.18)
 |
|
Optimized Daughter Spectrum (at Collision Energy 46eV)
 |
|
Transition 4: ES+, m/z 319.90 -> 178.16
Cone Voltage Optimization (m/z 319.90)
 |
|
Optimized MS Spectrum (at Cone Voltage 16V)
 |
|
Collision Energy Optimization (m/z 319.90 -> 178.16)
 |
|
Optimized Daughter Spectrum (at Collision Energy 32eV)
 |
|
Transition 5: ES+, m/z 319.90 -> 256.02
Cone Voltage Optimization (m/z 319.90)
 |
|
Optimized MS Spectrum (at Cone Voltage 16V)
 |
|
Collision Energy Optimization (m/z 319.90 -> 256.02)
 |
|
Optimized Daughter Spectrum (at Collision Energy 30eV)
 |
|