Method Development Report
| MS1 Resolution | 1.0 |
|---|
| MS2 Resolution | 0.75 |
|---|
| Calibration Method | C:\MassLynx\IntelliStart\Results\Quantitative\Calibration_20120326_1.cal |
|---|
| Tune Method | C:\MassLynx\Xevo TQ-S - Acquity UPLC Installation Project.pro\ACQUDB\SYSTEM2.ipr |
|---|
| Cone Voltage Range | 2 - 100 |
|---|
| Collision Energy Range | 2 - 80 |
|---|
| Lowest Fragment Mass | 40.00 |
|---|
| Excluded Losses | 18.00, 44.00 |
|---|
Date: Generated on Thu 29 Mar 2012 at 16:00
Results
IntelliStart generated the following experiments:
| MRM Experiment |
C:\MassLynx\Xevo TQ-S - Acquity UPLC Installation Project.pro\ACQUDB\URACIL_pos.exp |
IntelliStart found the following compounds:
| Compound |
Formula/Mass |
|
Parent m/z |
Cone Voltage |
Daughters |
Collision Energy |
Ion Mode |
| URACIL |
112 |
|
|
|
|
|
|
Compound
Transition 1: ES+, m/z 112.97 -> 84.06
Cone Voltage Optimization (m/z 112.97)
 |
|
Optimized MS Spectrum (at Cone Voltage 96V)
 |
|
Collision Energy Optimization (m/z 112.97 -> 84.06)
 |
|
Optimized Daughter Spectrum (at Collision Energy 14eV)
 |
|
Transition 2: ES+, m/z 112.97 -> 56.05
Cone Voltage Optimization (m/z 112.97)
 |
|
Optimized MS Spectrum (at Cone Voltage 96V)
 |
|
Collision Energy Optimization (m/z 112.97 -> 56.05)
 |
|
Optimized Daughter Spectrum (at Collision Energy 14eV)
 |
|