Method Development Report
| MS1 Resolution | 0.75 |
|---|
| MS2 Resolution | 0.75 |
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| Calibration Method | C:\MassLynx\IntelliStart\Results\Unit Mass Resolution\Calibration_20111216.cal |
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| Tune Method | C:\MassLynx\Xevo TQ-S - Acquity UPLC Installation Project.pro\ACQUDB\SYSTEM2.ipr |
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| Cone Voltage Range | 10 - 100 |
|---|
| Collision Energy Range | 2 - 80 |
|---|
| Lowest Fragment Mass | 40.00 |
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| Excluded Losses | 18.00 |
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Date: Generated on Wed 09 Jan 2013 at 13:58
Results
IntelliStart generated the following experiments:
| MRM Experiment |
C:\MassLynx\Xevo TQ-S - Acquity UPLC Installation Project.pro\ACQUDB\D12J2_neg.exp |
IntelliStart found the following compounds:
| Compound |
Formula/Mass |
|
Parent m/z |
Cone Voltage |
Daughters |
Collision Energy |
Ion Mode |
| D12ProstaglandinJ2 |
334.2 |
|
|
|
|
|
|
Compound
D12ProstaglandinJ2 |
(334.2) |
Transition 1: ES-, m/z 333.23 -> 233.10
Cone Voltage Optimization (m/z 333.23)
 |
|
Optimized MS Spectrum (at Cone Voltage 10V)
 |
|
Collision Energy Optimization (m/z 333.23 -> 233.10)
 |
|
Optimized Daughter Spectrum (at Collision Energy 8eV)
 |
|
Transition 2: ES-, m/z 333.23 -> 271.23
Cone Voltage Optimization (m/z 333.23)
 |
|
Optimized MS Spectrum (at Cone Voltage 10V)
 |
|
Collision Energy Optimization (m/z 333.23 -> 271.23)
 |
|
Optimized Daughter Spectrum (at Collision Energy 16eV)
 |
|
Transition 3: ES-, m/z 333.23 -> 189.11
Cone Voltage Optimization (m/z 333.23)
 |
|
Optimized MS Spectrum (at Cone Voltage 10V)
 |
|
Collision Energy Optimization (m/z 333.23 -> 189.11)
 |
|
Optimized Daughter Spectrum (at Collision Energy 18eV)
 |
|